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KMID : 0900919970210030247
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Korean journal of Animal Reproduction
1997 Volume.21 No. 3 p.247 ~ p.253
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In Vitro Fertilization and Development of In Vitro Matured Porcine Oocytes by Morphologically Normal Sperm Ratio of Epididymal Sperm and Frozen-Thawed Ejaculated Sperm
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Lee Hyun-Sook
Kim I. C.
Choi Dong-Yoon
Kim Eun-Young
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Abstract
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The objective of this study was to examine the fertilization and embryonic development rates of porcine oocytes matured in vitro according to the morphological normality of epididymal sperm in porcine IVF. The results obtained in this experiment were summarized as follows : 1. When the ratio of morphological normality of epididymal sperm was divided into the three groups with leq 10%, 10-30% and geq 50%, the fertilization and embryonic devel-opment rates of 능50% group (64%. 26%) w were significantly higher than those of other two groups (leq10%: 27%, 6% and 10-30%: 36%. 5%) (pleq50% group of morphologically normal epididymal sperm was adjusted to 100% (5 x 10^5 cells/ml), the fertilization a and development rates (63%, 27%) of epidymal sperm were similar to those of frozen-thawed ejaculated sperm (56%, 35%). 3. Also, when the fertilization and development rates of epididymal sperm were analyzed according to the oocyte : sperm ratio (1:6000, 1: 6650, 1: 7700, 1: 10000), the fertilization and development rates indicated high, in 1:6000 (68%, 32%) and 1:6650 (89%, 31%), the ratio of oocyte: sperm. Therefore, when the percentage of morphological normality of epididymal sperm is more than 50, the embryonic development a can be obtained similar to that of frozen thawed ejaculated sperm. Also, these result suggested that the morphological evaluation of normal sperm in porcine IVF using epididymal sperm sho비d be prerequisite for the more effective embryonic developm ment.
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KEYWORD
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Porcine, Epididymal/Ejaculated sperm, Embryonic development
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